Curriculum Vitae

Clyde H. Barlow

Title:
Faculty Member, The Evergreen State College
President, Barlow Scientific, Inc.

Education:
Eastern Washington State College    B.S., Chemistry    1968
Arizona State University Ph.D., Chemistry 1973
Johnson Research Foundation, Univ. of Penn. Postdoctoral Fellow, Medical Biophysics 1973-1974
National Institutes of Health, Univ. of Penn. Postdoctoral Fellow, Medical Biophysics 1974-1976

Employment and Experience:
Visiting Scientist    Dept. of Chemistry, Univ. of Washington    1984-1992
President Barlow Scientific, Inc. 1982-present
Member of the Faculty Chemistry and Biophysics, The Evergreen State College 1981-present
Research Assistant Professor Dept. of Surgery, Univ. of Penn. Medical School 1978-1981
Research Assistant Professor Dept. of Biochemistry/Biophysics, Univ. of Penn. 1977-1981
Research Associate Harrison Dept. of Surgical Research, Univ. of Penn. Medical School 1977-1978
Research Associate Johnson Research Foundation, Univ. of Penn. 1976-1977
Research Associate Dept. of Biochemistry, Colorado State Univ. 1973
Chemistry Instructor Medical Field Service School, Ft. Sam Houston, Texas 1966-1968

Honors:
Society of Plastic & Reconstructive Surgeons, Award of Merit (1980)

Societies:
American Society of Biological Chemists (1983); N.Y. Academy of Science (1984); Society for Applied Spectroscopy (1988); Biophysical Society (1995); Biomedical Engineering Society (1995)

Patents:
U.S. Patent No. 5,422,730, "Automated Optical Detection of Tissue Perfusion by Microspheres", June 1995.

Publications:
  • Barlow, C.H., Maxwell, J.C., Wallace, W.J. and Caughey, W.S.  (1973)  Elucidation of the mode of oxygen binding to hemoglobin by infrared spectroscopy. Biochem. Biophys. Res. Commun. 55:91-95.
  • Caughey, W.S., Barlow, C.H., O´Keeffe, D.H. and O´Toole, M.C.  (1973)  Spectroscopic studies of cis and trans effects in hemes and hemins. Ann. N.Y. Acad. Sci. 206:296-299.
  • Kubie, V.L., Anders, M.W., Engel, R.R., Barlow, C.H. and Caughey, W.S.  (1974)  Metabolism of dihalomethanes to carbon monoxide. I. In vivo studies, Drug Metabolism and Disposition 2:53-57.
  • Barlow, C.H.  (1974)  Spectroscopic probes of the oxygen binding sites of hemes and hemeproteins. Doctoral Dissertation, Arizona State University.
  • Maxwell, J.C., Volpe, J.A., Barlow, C.H. and Caughey, W.S.  (1974)  Infrared evidence for the mode of binding oxygen to iron of myoglobin from heart muscle. Biochem. Biophys. Res. Commun. 58:166-171.
  • Maxwell, J.C., Barlow, C.H., Spallholz, J.E. and Caughey, W.S.  (1974)  The utility of infrared spectroscopy as a probe of intact tissue: Determination of carbon monoxide in blood and heart muscle. Biochem. Biophys. Res. Commun. 61:230-236.
  • Fuchsman, W.H., Barlow, C.H., Wallace, W.J. and Caughey, W.S.  Novel heme: O2 bonding of possible relevance to oxygen utilizing hemeproteins. Biochem. Biophys. Res. Commun. 61:635-643.
  • Caughey, W.S., Barlow, C.H., Volpe, J.A. and Maxwell, J.C.  (1975)  Reactions of oxygen with hemoglobin, cytochrome c oxidase and other hemeproteins. Ann. N.Y. Acad. Sci. 244:1-9.
  • O'Keeffe, D.H., Barlow, C.H., Smythe, G.A., Fuchsman, W.H., Moss, T.H., Lilienthal, H.R. and Caughey, W.S.  (1975)  Magnetic and spectroscopic probes for FeOFe linkages in hemin systems. Bioinorganic Chemistry 5:125-148.
  • Barlow, C.H. and Chance, B.  (1976)  Ischemic areas in perfused rat hearts: Measurement by NADH fluorescence photography. Science 193:909-910.
  • Barlow, C.H., Ohlsson, P.I., and Paul, K.G.  (1976)  Infrared spectroscopic studies of carbonyl horseradish peroxidases. Biochemistry 15:2225-2229.
  • Gadian, D.G., Holt, D.I., Radda, G.K., Seeley, P.J., Chance, B. and Barlow, C.H.  (1976)  Phosphorus nuclear magnetic resonance studies on normoxic and ischemic cardiac tissue. Proc. Natl. Acad. Sci., USA 73:4446- 4448.
  • Franke, H., Barlow, C.H., and Chance, B.  (1976)  Oxygen delivery in perfused rat kidney: NADH fluorescence and renal functional state. Amer. J. Physiol. 231:1082-1089.
  • Steenbergen, C., DeLeeuw, G., Barlow, C.H., Chance, B. and Williamson, J.R.  (1977)  Heterogeneity of the hypoxic state in perfused rat heart. Circ. Res. 41:606-615.
  • Barlow, C.H., Harken, A.H. and Chance, B.  (1977)  Evaluation of cardiac ischemia by NADH fluorescence photography. Ann. Surg. 186:737-740.
  • Harken, A.H., Barlow, C.H. and Chance, B.  (1977)  Evaluation of myocardial oxygen supply/demand by NADH fluorescence photography. Surg. Forum 28:271-274.
  • Williamson, J.R., Steenbergen, C., Rich, T., DeLeeuw, G., Barlow, C.H. and Chance, B.  (1977)  The nature of ischemic injury in cardiac tissue. In Pathophysiology and Therapeutics of Myocardial Ischemia, (eds. Leffer, A.M., Kelliher, G.J. and Rovetto, M.J.), pp. 193-225. Spectrum Publications Inc.
  • Chance, B., Barlow, C.H., Haselgrove, J., Nakase, Y., Quistroff, B., Matschinsky, F. and Mayevsky, A.  (1978)  Microheterogeneities of redox states of perfused and intact organs. In Microenvironments and Metabolic Compartmentation, (eds. Srere, P.A. and Estabrook, R.W.), pp. 131-148. Academic Press, New York.
  • Harden, W.R., Simson, M.B., Barlow, C.H., Soriano, R. and Harken, A.H.  (1978)  Display of epicardial ischemia by reduced nicotinamide adenine dinucleotide fluorescence photography, electron microscopy, and ST segment mapping. Surgery 83:732-740.
  • Reed, G.H., Barlow, C.H. and Burns, R.A.  (1978)  Investigation of anion binding in transition state analogue complexes of creatine kinase by infrared spectroscopy. J. Biol. Chem. 253:4153-4158.
  • Powers, L., Chance, B., Smith, J., Barlow, C.H. and Leigh, J.  (1978)  Identification of the nature of the copper atoms in cytochrome oxidase by X-ray photon absorption. In Frontiers of Biological Energetics: From Electrons to Tissues, (eds. Dutton, P.L., Leigh, J.S. and Scarpa, A.), pp. 863-872. Academic Press, New York.
  • Bashford, L., Barlow, C.H., Chance, B., Smith, J., Silberstein, B. and Rehncrona, S.  (1978)  Some properties of the extrinsic probe, Oxonol-V, in tissues. In Frontiers of Biological Energetics: From Electrons to Tissues, (eds. Dutton, P.L., Leigh, J.S. and Scarpa, A.), pp. 1305-1312. Academic Press, New York.
  • Austin, G., Jutzy, R., Chance. B. and Barlow, C.H.  (1978)  Noninvasive monitoring of human brain oxidative metabolism. In Frontiers of Biological Energetics: From Electrons to Tissues, (eds. Dutton, P.L., Leigh, J.S. and Scarpa, A.), pp. 1445-1456. Academic Press, New York.
  • Haselgrove, J., Barlow, C.H., Chance, B., Joyce, E., Kanamuller, H. and Bruchner, M.  (1978)  Three dimensional display of the ischemic region of a rat heart. In Frontiers of Biological Energetics: From Electrons to Tissues, (eds. Dutton, P.L., Leigh, J.S. and Scarpa, A.), pp. 1515-1522. Academic Press, New York.
  • Barlow, C.H., Harken, A.H., Simson, M.B., Harden, W., Rastegar, H. and Chance, B.  (1978)  Resolution of ischemic borderzone by NADH fluorophotography in isolated perfused rabbit hearts. In Frontiers of Biological Energetics: From Electrons to Tissues, (eds. Dutton, P.L., Leigh, J.S. and Scarpa, A.), pp. 1533-1540. Academic Press, New York.
  • Harken, A., Barlow, C.H., Harden, W. and Chance, B.  (1978)  Two and three dimensional display of myocardial ischemic "border zones" in dogs. Am. J. Cardiol. 42:954-959.
  • Williamson, J.R., Steenbergen, C., Deleeuw, G. and Barlow, C.H.  (1978)  Control of energy production in cardiac muscle: Effects of ischemia in acidosis. In Heart Function and Metabolism Vol. 11 of Recent Advances in Studies on Cardiac Structure and Metabolism, (eds. Kobayashi, T., Sano, T. and Dhalla, N.S.), pp.521-531. University Park Press, Baltimore.
  • Weiss, J.P., Barlow, C.H. and Chance, B.  (1978)  Pentobarbital induced reduction of pyridine nucleotide measured by surface fluorometry in perfused rat heart. Biochemical Pharmacology 27:1510-1511.
  • Barlow, C.H., Harden, W.R., Harken, A.H., Simson, M.B., Haselgrove, J.C., Chance, B., O'Connor, M. and Austin, G.A.  (1979)  Fluorescence mapping of mitochondrial redox changes in heart and brain. Crit. Care Med. 7:402-406.
  • Barlow, C.H. and Erecinska, M.  (1979)  Orientation of the NO ligand of cytochrome a3 in nitrosyl cytochrome c oxidase. FEBS Lett. 98:9-12.
  • Barlow, C.H., Chance, B., Harden, W., Simson, M. and Harken, A.  (1979)  Spectroscopic mapping of oxygen supply-demand in heart. In Biochemical and Clinical Aspects of Oxygen, (ed. Caughey, W.S.), pp. 845-857. Academic Press, New York.
  • Harden, W.R., Barlow, C.H. and Harken, A.H.  (1979)  Epicardial NADH fluorescence in altered mitochondrial redox states. J. Surg. Res. 27:156-162.
  • Simson, M.B., Harden, W.R., Barlow, C.H. and Harken, A.H.  (1979)  Epicardial ischemia as delineated by epicardial ST segment mapping and NADH fluorescence photography. Am. J. Cardiol. 44:263-269.
  • Powers, L., Blumberg, W.E., Chance, B., Barlow, C.H., Leigh, J.S., Smith, J., Yonetani, T., Vik, S. and Peisach, J.  (1979)  The nature of the copper atoms of cytochrome c oxidase as studied by optical and X-ray absorption edge spectroscopy. Biochem. Biophys. Acta 546:520-535.
  • Simson, M.B., Harden, W.R., Barlow, C.H. and Harken, A.H.  (1979)  Visualization of the distance between perfusion and anoxia along an ischemic border. Circulation 60:1151-1155.
  • Harden, W.R., Barlow, C.H., Simson, M.B. and Harken, A.H.  (1979)  Temporal relation between onset of cell anoxia and ischemia contractile failure: Myocardial ischemia and left ventricular failure in isolated, perfused rabbit heart. Am. J. Cardiol. 44:741-746.
  • Harden, W.R., Simson, M.B., Barlow, C.H. and Harken, A.H.  (1979)  Successful pharmacological modification of myocardial ischemia. Surg. Forum 30:260-262.
  • Franke, H., Barlow, C.H. and Chance, B.  (1980)  Fluorescence of pyridine nucleotide and flavoproteins as an indicator of substrate oxidation and oxygen demand of the isolated perfused rat kidney. Int. J. Biochem. 12:269-275.
  • Franke, H., Barlow, C.H. and Chance, B.  (1980)  Surface fluorescence of reduced pyridine nucleotide of the perfused rat kidney: Interrelation between metabolic and functional states. Contr. Nephrol. 19:240-247.
  • Harden, W.R., Barlow, C.H., Simson, M.B. and Harken, A.H.  (1980)  Heterogeneity of the coronary microcirculation during low flow ischemia: A model for the heart in shock. Advances in Shock Research 3:57-63.
  • Silverman, D.G., Larossa, D.D., Barlow, C.H., Bering, T.G., Popky, L.M. and Smith, T.C.  (1980)  Quantification of tissue fluorescein delivery and prediction of flap viability with the fiberoptic dermofluorometer. Plastic and Reconstructive Surgery 66:45-553.
  • Chance, B., Barlow, C.H., Haselgrove, J., Nakase, Y., Takeda, H., Mayevsky, A., Fischetti, R., Graham, N. and Sorge, J.  (1980)  Heterogeneity of oxygen delivery in normoxic and hypoxic states: A fluorometer study. Am. J. Physiol. 235:809-H820.
  • Haselgrove, J.C., Barlow, C.H. and Chance, B.  (1980)  The 3D distribution of metabolic states in the gerbil brain during the course of spreading depression. In Cerebral Metabolism and Neural Function, (eds. Passonneau, J., Hawkins, R. and Welsh, F.), pp. 72-76. Williams and Williams, Baltimore.
  • Harken, A.H., Simson, M.B., Haselgrove, J., Wetstein, L., Harden, W.R. and Barlow, C.H.  (1981)  Early ischemia after complete coronary ligation in the rabbit, dog, pig and monkey. Am. J. Physiol. 241:H202- H210.
  • Wetstein, L., Nussbaum, M.S., Barlow, C.H., Harden, W.R., Simson, M.B., Slagle, B. and Harken, A.H.  (1981)  Decrease in acute myocardial ischemia by hyaluronidase in isolated, perfused rabbit hearts. J. of Surg. Res. 30:489-496.
  • Bashford, C.L., Barlow, C.H., Chance, B., Haselgrove, J. and Sorge, J.  (1982)  Optical measurements of oxygen delivery and consumption in gerbil cerebral cortex. Am. J. Physiol. 242:C265-C271.
  • Wetstein, L., Simson, M.B., Haselgrove, J., Barlow, C.H. and Harken, A.H.  (1982)  Mechanism of action of hyaluronidase in decreasing myocardial ischemia post coronary occlusion in the isolated perfused rabbit heart. Amer. Heart J. 104:529-536.
  • Chance, B., Eleff, S., Leigh, J.S., Barlow, C.H., Ligetti, L. and Gyulai, L.  (1983)  Phosphorus NMR. In Nuclear Magnetic Resonance (NMR) Imaging, (eds. Partain, C.L., James, Jr., A.E. and Price, R.R.), pp. 399-415. W.B. Saunders Co., Philadelphia.
  • Wetstein, L., Rastegar, H., Barlow, C.H. and Harken, A.H.  (1984)  Delineation of myocardial ischemia in an isolated blood-perfused rabbit heart preparation. J. Surg. Res. 37:285-289.
  • Gyulai, L., Bolinger, L., Leigh, J.S., Barlow, C.H. and Chance, B.  (1984)  Phosphorylethanolamine--the major constituent of the phosphomonoester peak observed by 31P-NMR on developing dog brains. FEBS Lett. 176:137-142.
  • Schnall, M.D., Barlow, C., Subramanian, V.H., and Leigh, J.S.  (1986)  Wireless implanted magnetic resonance probes for in vivo NMR. J. Magn. Reson. 68:161-167.
  • Blum, H., Summers, J.J., Schnall, M.D., Barlow, C.H., Leigh, J.S., Chance, B. and Buzby, G.P.  (1986)  Acute intestinal ischemia studies by phosphorus nuclear magnetic resonance spectroscopy. Annals of surgery 204:83-88.
  • Blum, H., Summers, J.J., Schnall, M.D., Barlow, C.H., Leigh, J.S., Buzby, G.P. and Chance, B.  (1985)  Intestinal ischemia studied by 31P NMR. New Metabolic Res. 2(1):4-6.
  • Schnall, M.D., Bolinger, L., Renshaw, P.F., Haselgrove, J.C., Subramanian, V.H., Eleff, S.M., Barlow, C.H., Leigh, J.S. and Chance, B.  (1987)  Multinuclear MR imaging: A technique for combined anatomic and physiologic studies. Radiology 162:863-866.
  • Barlow, C.H., Rorvik, D.A. and Kelly, J.J.  (1988)  Epicardial oxygen/metabolism by Pd porphyrin and NADH imaging. J. Cell Bio. 107:213a.
  • Kelly, J.J., Barlow, C.H., Jinguji, T.M. and Callis, J.B.  (1989)  Prediction of gasoline octane numbers by near infrared spectroscopy in the spectral range 680-1235 nm. Anal. Chem. 61:313-320.
  • Phalen, M.K., Barlow, C.H., Kelly, J.J., Jinguji, T.M. and Callis, J.B.  (1989)  Measurement of caustic and caustic brine by spectroscopic detection of the hydroxide ion in the wavelength range 700-1150 nm. Anal. Chem. 61:1419-1424.
  • Barlow, C.H. and Kelly, J.J.  (1989)  Metabolic spectroscopy for monitoring electromagnetic medical techniques. In Emerging Electromagnetic Medical Technology, (eds. O'Connor, M.E. and Monahan, M.E.), pp. 103-124. Springer Verlag, New York.
  • Rorvik, D.A., Kelly, J.J., Burns, D.H. and Barlow, C.H.  (1989)  Imaging cytochrome oxidation in hypothermically stored rat kidneys. IEEE/EMBS 11(2):622-623.
  • Kelly, J.J., Rorvik, D.A., Richmond, K.N. and Barlow, C.H.  (1989)  Videofluorometer for imaging tissue metabolism. Rev. Sci. Instr. 60(11):3498-3502.
  • Tabbutt, F.C., Kelly, J.J., Cole, R.M., Middendorf, D.H. and Barlow, C.H.  (1989)  Introductory Laboratory: A new exploration. J. Chem. Ed. 66(11):940-943.
  • Barlow, C.H., Burns, D.H. and Callis, J.B.  (1989)  Breast biopsy analysis by spectroscopic imaging in photon migration in tissue. In Photon Migration in Tissues, (ed. Chance, B.), pp.111-120. Plenum Press, NY.
  • Kelly, J.J., Kelly, K.A. and Barlow, C.H.  (1991)  Myoglobin oxygen binding curves determined by phosphorescence quenching. Appl. Spectrosc. 45:1177-1182.
  • Barlow, C.H., Kelly, K.A. and Kelly, J.J.  (1992)  Simultaneous determination of hemoglobin and myoglobin oxygen binding curves by spectral curve fitting. Appl. Spectrosc. 46:758-763.
  • Kelly, J.J., Kelly, K.A. and Barlow, C.H.  (1995)  Tissue temperature by near-infrared spectroscopy. In Optical Tomography, Photon Migration, and Spectroscopy of Tissue and Model Media, (ed. Chance, B and Alfano, R.R.) SPIE, 2389:818-828.
  • Kelly, J.J., Holloway, G.A.,Jr., Rorvik, D.A., Reinbold, T.R. and Barlow, C.H. Skin flap circulation: A comparison of fluorescein videofluorometry and laser Doppler detection. Submitted to Plastic and Reconstructive Surgery.
  • Conley, K.E., Rorvik, D.A., Barlow, C.H., Kelly, J.J. and Kushmerick, M.J. Digital imaging: Spatial resolution of NADH fluorescence in heart. In preparation for submission to American Journal of Physiology.
  • Barlow, C.H., Kelly, K.A., Bailey, E. and Kelly, J.J.  (1998)  Reflectance measurement of heart muscle oxygenation.  In Oxygen Transport to Tissue XX, (eds. Hudetz, A.G. and Bruley, D.), Plenum Press.
  • Kelly, J.J., Barlow, C.H., Kelly, K.A. and Ewen, J.  (1998)  Sublobular distribution of cytochromes in cold-stored rat liver.  In Oxygen Transport to Tissue XX, (eds. Hudetz, A.G. and Bruley, D.), Plenum Press.
  • Barlow, C.H., Rorvik, D.A. and Kelly, J.J.  (1998)  Imaging epicardial oxygen. Annals of Biomedical Engineering 26(1):76-85.
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Last modified: 02/06/1999