Videofluorometer For Imaging Tissue Metabolism

Jeffrey J. Kelly, Dawn A. Rorvik, Keith N. Richmond, and Clyde H. Barlow

Barlow Scientific, Inc., 6307 Tamoshan Dr. NW, Olympia, WA 98502
The Evergreen State College, Olympia, WA 98505

J.J. Kelly, D.A. Rorvik, K.N. Richmond and C.H. Barlow  (1989)  Videofluorometer for imaging tissue metabolism. Rev. Sci. Instrum. 60(11):3498-3502. A videofluorometer is described that directly acquires digital metabolic images of reduced nicotinamide adenine dinucleotide (NADH) fluorescence in tissue. NADH fluorescence provides an intrinsic indicator of the state of tissue mitochondrial oxidative metabolism. The device combines a computer-controlled fluorescence excitation system with digital image acquistion to quantify tissue bioenergetics in both spatial and time domains. Localized ischemia following coronary artery ligation in a perfused rat heart (model for a coronary artery occlusion heart attack) is used as an example to demonstrate the capabilities of the system. This videofluorometer permits monitoring changes in physiological state of organs and tissue without interfering with tissue metabolism. The digital nature of the acquired image allows detailed analysis of physiological features and their time dependence.

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Last modified: 01/08/2000