2003 Meeting, Olympia, WA | 2000 Meeting, Montreal, Canada

Evergreen State College, Olympia, WA 98505

Elizabeth Kutter -- 360 867 6099; t4phage@evergreen.edu

Rebecca MacDougal, Meeting Organizational Assistant, the students and faculty from the Evergreen Phage Lab and the Eliava Institute, and the members of the Organizing Committee

Contributors:

Note that talks presented at the meeting are not considered formal publications, and often reflect works in progress. Thus, anything from an abstract or presentation can only be cited, as personal communication, with the express permission of the author, and the full abstracts will not be posted here on the web page but must be obtained from the authors. A listing of contact information for attendees is also posted here on the web site.

1. Viruses and Nutrient Cycles in the Sea. Curtis Suttle, UBC

2. Contribution of Latent Period to Phage Population Growth. Stephen T. Abedon, Ohio State

3. Comparative Genomics of P2-Related Phages: Sequence Analysis of Wf and PSP3. Gail E. Christie¹, Ping Xu¹, Patrik Vitazka¹, Gregory A. Buck¹, Fredric Bloom², Dominic Esposito², and Brian Schmidt² Virginia commonwealth U, Invitrogen Corp.

4. Comparative Genomics Reveals Close Genetic Relationships Between Phages from Dairy Bacteria and Pathogenic Streptococci: Evolutionary Implications for Prophage-Host Interactions. Frank Desiere, Marco Ventura, Carlos Canchaya, William McShan and Harald Bruessow 3, Nestle

5. a. Effects of SPO1 Host-takeover Genes on Bacterial Physiology and Viability. Charles Stewart, Bati Myles, Katie Cox, Lea Ann Chen, Allegra Petti and Aruna Sampath. Rice University.

b. Role of SPO1 Genes 44, 50, and 51 in Host-takeover and Bacterial Cell Death. Aruna Sampath, Melanie Monroe-Augustus, Katie Cox, and Charles Stewart, Rice U

6.*Roles in Mutagenesis and Horizontal Gene Transfer of Two Recombination-Dependent DNA Replication Pathways of Phage T4. Gisela Mosig, Nancy Colowick, John Gewin, Daniel Vo, and Richard Calendar – Vanderbilt, Berkeley

7. Sequencing genomes of bacteriophages that resemble T4. Tulane University (Vasiliy Petrov, Dawn Chin, Jim Nolan and Jim Karam,), New Orleans, LA 70112 and Centre Nationale de Recherche Scientifique, UMR 5100 (Carine Desplats, Françoise Tétart, Andrey Laterov and H. M. Krisch), Toulouse, France.

8. Genetic and evolutionary analyses of the gene transfer agent (GTA) of Rhodobacter capsulatus. Andrew S. Lang -- University of British Columbia

9. Bacteriophage Genus Names. Hans-W. Ackermann, Laval University, Quebec

10. Association Equilibrium and crystal structure of the Tail Lysozyme (gp5) – gp27 Complex of Bacteriophage T4. Shuji Kanamaru1), Petr Leiman1), Yoko Takeda2), Michael G. Rossmann1), and Fumio Arisaka2), Purdue & Tokyo Tech

11. The structure and evolution of the fibritin protein (gp wac) in the T4-type phages. Letarov & H. Krisch, France

12. Interactions between the packaged f X174 genome and the capsid’s inner surface may influence the final stages of morphogenesis and the virion. Susan Hafenstein and Bentley A. Fane, U of Arizona

13. Structure-function analysis of the NH₂ terminus of the øX174 Internal scaffolding protein. C. R. Novak and B. A. Fane, U of Arizona

14. Structure-function anaylses of the øX174 external scaffolding protein using chimeric proteins. B. A. Fane and A. D. Burch, U of Arizona

15. Staphylococcal bacteriophage: biological characterization and clinical results of its therapeutic usage. R.Adamia, L.Kvachadze, M.Kutateladze, N.Balarjishvili, (Eliava Institute, Tbilisi)

16. The Challenge of Pseudomonas aeruginosa Biofilms in the Lungs of Cystic Fibrosis Patients: Potential Alternate Therapies. Kalai Mathee. Florida International University

17. The application of specific bacteriophages in the treatment of infections of patients with cystic fibrosis. B. Weber-Dąbrowska ¹, M. Mulczyk ¹, M. Lusiak ¹, A. Górski ¹, A. Minarowska ², M. Kaczmarski ², A. Pogorzelski. Wroclaw, Poland

18. Studies of Phage Infection in Stationary Phase and other Environmentally Interesting Conditions. Elizabeth Kutter, Chelsea Thomas, Lex Ball, Yvonne Roberts and Liana Gachechiladze (TESC & Tbilisi)

19. Anaerobic Fermentation and Respiration in E. coli. Diane Nelsen, Robert Rutherford, Andrew Brabban. Evergreen State College

20. Infection of E. coli with T-even Phages under conditions of anaerobic fermentation and respiration. Peter Varey, Costanzo Alione, Matthew Robison, Chelsea Thomas, Sara Larson, Rebecca MacDougal, Andrew Brabban, Elizabeth Kutter. Evergreen State College

21. The inhibition of Staphylococcus aureus bacteriophage activity by raw bovine milk. J. J. Gill¹, J. Pacan², D. Lepp², M. Griffiths¹, M. P. Sabour² , Guelph, Ontario

22. Development of Lysogenic Phage for Phage Therapy. M. E. Rapson¹, L. P. Glancy¹, D. A. Hodgson¹, R. J. Sharp² and N. H. Mann¹, England

23. Nonproliferation through Science Cooperation, David C. Allen and Elizabeth C. Scharl

U.S. Department of State, International Science and Technology Center

24. Agricultural applications of phages: an overview – Alexander Sulakvelidze, Intralytix

25. Bacteriophage therapy as a potential treatment for the disease furunculosis in salmonid fishes. Theresa Nation, Yvonne Roberts, Elizabeth Kutter. Evergreen State College

26. Viruses in a deep-sea hydrothermal plume. Alice Ortmann and Curtis Suttle, U. of British Columbia

27. Seasonal Abundance and Host Ranges of Phages Infecting Vibrio parahaemolyticus in Oysters, Sediments, and Seawater from the Strait of Georgia, British Columbia. André M. Comeau¹ and Curtis A. Suttle^1,2, UBC

28. Prochlorococcus-Infecting Marine Cyanophages. Sullivan, M.B, Waterbury, J.B., Chisholm, S.W, MIT/WHOI

29. Molecular Examination of Cyanomyovirus Communities in British Columbia Coastal Waters. Cindy M. Frederickson and Curtis A. Suttle., UBC

30. A possible role for RNA in determining replisome specificity of a DNA polymerase: Studies with gp43 of the T4-related phages. Vasiliy Petrov, Dawn Chin and Jim Karam.,Tulane

31. The DNA polymerase (gp43) of bacteriophage RB49. Karam lab, Tulane University, New Orleans, LA (Vasiliy Petrov, Dawn Chin, Sandy Ng and Jim Karam) and Krisch lab, CNRS-UMR5100, Toulouse, France (Carine Desplats, Françoise Tétart and Henry Krisch).

32. 3¹ ­ 5¹ Exonucleolytic Activity of DNA Polymerases: Structural Features that Allow Kinetic Discrimination Between Ribo- and Deoxyribonucleotide Residues. T.-C. Lin, Chun Xia Wang, Catherine M. Joyce, and William H. Konigsberg, Yale University

33. How Well Does a DNA Polymerase Achieve Fidelity in Vitro Versus In Vivo? Anna Bebenek,¹ Holly Kloos Dressman,¹ Geraldine T. Carver,¹ William H. Konigsberg,² Jim D. Karam³ and John W. Drake¹, Yale & Tulane.

34. Binding of T4 RepEB Protein to a Sequence Upstream of the Primer Promoter is Required to Convert Transcripts into Primers for Leading Strand DNA Synthesis at T4 Origin E. Gisela Mosig, Rekha Mody, Laura Davenport, Alex Harvey and Rita Vaiskunaite, Vanderbilt.

35. Protein-Protein Interactions in the T4 dNTP Synthetase Complex. Christopher K. Mathews, Linda J. Wheeler and Rongkun Shen. Oregon State University

36. The pattern of DNA recombination during repair of site-specific double-stranded breaks in normal T4 infection as evidenced from recombinational data. Victor Shcherbakov, Igor Granovsky, Lidiya Plugina, Tamara Shcherbakova, Svetlana Sizova. Chernogolovka, Russia

37. a. Present Status of Phage T5 Sequencing. Vladimir N. Ksenzenko, Alexander V. Kaliman.

b. Phage T5 Encoded Site-Specific Endonucleases: Identification, Gene Cloning, Biochemical Properties. Vladimir N. Ksenzenko¹, Natalya V. Akulenko², Alexander V. Kaliman¹, Natalya M. Shestakova¹, Antomina I .Krutilina¹, Svetlana V. Ustanina¹, Sergey G. Mayorov¹, Alexander A. Kanapin³, and Michael G. Shlyapnikov², Pushchino, Russia

38. Gene insertion and replacement in S. pombe mediated by the Streptomyces bacteriophage f C31 site-specific recombination system. Lynn Thomason¹, Richard Calendar¹ and David Ow². ¹University of California, Berkeley, and ²USDA Plant Gene Expression Center, Albany, CA.

39. Genomics Of Listeria Phages, And Construction Of Site-Specific Integration Vectors. Richard Calendar1, Peter Lauer1, Daniel Portnoy1, Elke Sattelberger2, Markus Zimmer2, Nora Chow1, Ross B. Inman³, and Martin J. Loessner1,2. Berkeley and Munich

40. Lysis of Listeria monocytogenes cells by phage-encoded endolysins and holins: Small proteins of many uses ! Martin J. Loessner , Munich

41. Utilizing Bacteriophage Lysins for the Diagnosis and Treatment of Infectious Diseases Associated with the Carrier State. Daniel Nelson, Rockefeller

42. Study of the peculiarities of the phage - host- cell interaction and DNA ejection processes in different conditions on the model of Cb and Un phages. E. Jaiani*, M. Tediashvili*, T. Mdzinarashvili, T. Eliashvili*, N.Janelidze* , N. Chanishvili*, D. Tushishvili*, A.Ivanova, G. Mrevlishvili. Tbilisi

43. Characterization of Microviridae infecting obligate intracellular parasitic bacteria. Karie Brentlinger¹, I. Clarke², M. Agbandge McKenna³, R. McKenna³, and B. A. Fane¹. U of Arizona & Florida.

44. Bacteriophage f 3626 from Clostridium perfringens: Isolation, characterization, and complete nucleotide sequence. Markus Zimmer, Siegfried Scherer, and Martin J. Loessner, Freising, Germany.

45. PY54, a linear plasmid prophage of Yersinia enterocolitica with covalently closed ends. Stefan Hertwig¹, Iris Klein¹, Rudi Lurz² and Bernd Appel¹, Berlin, Germany.

46. The characterization of gene products and genetic elements involved in the genetic switch of the temperate Lactococcus lactis phage f LC3. Janet Martha Blatny*, Per Arne Risøen, Ellen Maryann Rosenhave, Merete Lunde and Ingolf F. Nes., Norway.

47. The effect of growth conditions on the lytic versus lysogenic life cycle of the temperate lactococcal bacteriophage f LC3 monitored by real time quantitative PCR. Merete Lunde*, Janet Martha Blatny and Ingolf F. Nes., Norway.

48. Analysis of the genetic switch region in the temperate Streptococcus thermophilus phage Sfi21 and isolation of virulent phages under repressor selection. Anne Bruttin, Sophie Foley and Harald Bruessow, Nestle

49. The Immunobiology of Chlamydia pneumoniae Bacteriophage ΦCpn1. Karunakaran, P., Blanchard, J.F., Lu, H., Shen, C., and Brunham, R.C. University of British Columbia Centre for Disease Control

50. Evolution Of Phage Classification And Nomenclature. Jack Maniloff, University of Rochester, NY

Science and Public Policy: Experience of an AAAS Intern at ISTC – Elizabeth Scharl, ISTC

Summary of meeting highlights -- Chris Mathews

Discussion of phage nomenclature issues posed by Maniloff and Ackermann

Steve Abedon’s Phage Ecology website (www.phage.org) is serving an important and expanding function in awareness about many key phage issues, and our website, www.evergreen.edu/user/T4, focussed particularly on phage therapy issues, is drawing a lot of response, as is Eric Miller’s PhagePage – how can we make even better use of the web?

There is a new explosion of interest in phage, at many levels, from basic biology to ecology to applications. Ry Young is exploring the possibility of a Keystone symposium on The New Age of Phage: Evolution, Health, Ecology and Biotechnology – possibly in the winter of 2002. Mike DuBow is suggesting the possibility of some sort of phage society, possibly with a journal, as well as of regular phage meetings, which might alternate between the two coasts or occasionally abroad. The morphogenesis meeting is drawing in a broader structural phage-protein presence. There seems to also be an increasingly strong phage presence at other meetings, making early communication and integrated planning even more important. For example, next July the International Virology meeting in Paris will have 3 oral phage sessions, as well as, hopefully, a lot of posters, and there is discussion of having a miniphage meeting afterwards, possibly at the Pasteur institute or somewhere in the countryside.

1. Evolution of bacteriophage life history traits. Ing-Nang Wang, Texas A&M

2. Bacteriophages Of Vibrio Harveyi. Barbara Chang, Louise Thatcher, Paul Hinkley, Andre Crozier & David Sutton, Australia

3. Structural Analysis of the Tail Completion Proteins, P15 and P3, of Phage T4. Zhao Li¹⁾, Shuji Kanamaru²⁾, Chaidirek Chartree’chalerm¹⁾, Michael G. Rossmann²⁾, and Fumio Arisaka, Tokyo Tech & Purdue U.

4. Phage induction by mating in Bacillus thuringiensis serovar israelensis. Kohzo Kanda, Fumio Kato and Akira Murata, Saga U.

5. Comparison of in vitro susceptibility of Ps. aeriginosa strains towards antibiotics and specific phages. Glonti T., Lashkhi N., Tediashvili M., Kutateladze M., Balarjishvili N., Nuralova T., Chanishvili N., George Eliava Institute of Bacteriophage, Microbiology and Virology, Tbilisi, Georgia

6. Immunochemical Analysis of Lysates of Shigella flexneri by Specific Monoclonal and Polyvalent commercial Dysentery Phages. Ketevan Liana Gachechiladze. Tbilisi

7. Proteus Phage –029 and P113: A Comparative Study of Host Range, Antigenic Determinants and Structural Variations. Chanishvili Z.D, Chanishvili T.G., Cholokashvili N.A. Dsuliashvili M.A. and Gachechiladze Liana K. Tbilisi

8. Antibacterial activity of Bacillus amyloliquefaciens phage endolysin. Masatomo MORITA, Yasunori TANJI, Yuji ORITO and Hajime UNNO, Tokyo Tech.

9. Genetic Analysis of Host-takeover in SPO1 infection of B. subtilis. Aruna Sampath, Bati Myles, Katie Cox, Lea Ann Chen, Allegra Petti and Charles Stewart., Rice U

10. Plasmid-encoded Phage Resistance of Silage-Making Lactobacilli. Katsumi Doi, Tomoko Eguchi, Takashi Nishiyama, Yousuke Nishizaki, Sadahiro Ohmomo*, and Seiya Ogata , Kyushu U, Japan

11. Application of Polyvalent Salmonella Bacteriophages Against S. typimurium and S. pulurum Infections in Poultry Farms. Tato Gabisonia and Lamara Chanishvili., Tblisi

12. Isolation and Characterisation of Campylobacter Bacteriophage from Free-Range Chicken Farm. Catherine M Loc-Carrillo, Phillippa Connerton, Christine Dodd, Cath Rees and Ian Connerton., UK

13. Characterization of Virulent Coliphage Specific for Escherichia coli O157:H7. Yasunori Tanji, Masatomo Morita, Katsunori Mizoguchi, Tetsuya Akitsu, and Hajime Unno, Tokyo Tech

14. Biological Features of E. coli Bacteriophages and Their Usage against Calf E. coli Infections. T. Gabisonia and I. Kharabadze,

15. Use of Bacteriophage as Therapy for Disease Caused by Vibrio vulnificus in a Mouse Model and for Decontamination of Experimentally Infected Oysters. Karen Cerveny, M. A., Eric Wilkenning, A.A., Paul Gulig, Ph.D. and Donna Duckworth, Ph.D. U. of Florida, Gainesville

16. Lysogenic Phages of Clostridium difficile. Shan Goh*1, Thomas Riley1,2, Barbara Chang1

17. Transcription map of the temperate Streptococcus thermophilus phage Sfi21 Marco Ventura and Harald Bruessow. 2

18. Preliminary characterization of Myovirus LP65 isolated from Lactobacillus plantarum, a Gram-positive lactic acid bacterium. Sandra Chabani-Chennoufi and Harald Bruessow. 4

19. Filamentous Phages from Xanthomonas Require Type IV Pili for Infection. Yi-Hsiung Tseng, Nien-Tsung Lin, Ya-Wen Ke, Ming-Ren Yen, Mong-Chuan Lee, Tzu-Ching Lee and Miao-Yi Hsiao

20. Evaluation of different ways of introduction of therapeutic phages. N.P.Efimova, M.A.Kameneva, V.I.Reshetnikov, E.V.Funkner, BIOMED, Perm, Russia

21. Experience of Application of Pentophage in Obstetrics, PADRUL M.М, TRUSHKOV A.G., Perm, Russia

22. The Bacteriophage Therapy of Mastitis in Cows,caused by Staphylococcus and Streptococcus Infections. Tato Gabisonia. Eliava Institute of Bacteriophages, Tbilisi.

23. Biological Properties of Some Freshly Isolated Strains of Pseudomonas and Selection of the Active Phages Against Them. T.Suladze, M.Darsavelidze, M.Elizbarashvili (G.Eliava Institute of Bacteriophage, Microbiology and Virology, Georgian Academy of Sciences, Tbilisi, Georgia)

24. Host Range and Growth Characteristics of Two Novel Coliphages. Chelsea Thomas, Sarah Larson and Elizabeth Kutter

25. Biological characterization of two Pseudomonas aeruginosa phages. N.Balarjishvili, M.Kutateladze, S.Gurgenidze, L.Kvachadze, N.Chanishvili, R.Adamia, Tbilisi

26. The Characterization of a Unique Mutant of T4 Topoiosomerase That is Hypersensitive To a Broad Range of Cleavage-Inducing Antitumor Agents. E. O’Reilly and K. Kreuzer. Dept. of Microbiology, Duke University Medical Center

27. Bacteriophages as an alternative treatment for antibiotic resistant and EHEC E.coli infections. Wangjianyang. Henan provincial health & Anti-epidemic center. No.47,Weiwu Road,450003.Zhengzhou city. Henan province.P.R .China